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SDS-PAGE and Western blot analyses of the purified recombinant proteins with Ni-NTA. The presence of single bands in SDS-PAGE were indicatives of purified rEspC/EspB fusion protein (A), rEspC (C), and rEspB (E) proteins. Western blotting with anti-His antibodies confirmed the total expressions of the above-mentioned recombinant proteins (B, D, F), respectively.
