Figure 6.

SV2A R383Q cannot rescue Syt1 expression in SV2A-depleted cells. Cultured hippocampal neurons were transfected with SV2A shRNA and either an empty vector (mCerulean, mCer), wild-type (WT) SV2A-mCer, or R383Q SV2A-mCer at 7–9 DIV. After 13–15 DIV, neurons were fixed and immunostained for GFP, Syt1, and Synaptophysin (Syp). A, Representative images are displayed with arrowheads highlighting transfected nerve terminals (identified using Syp immunostaining). Scale bar = 10 μm. B, Quantification of Syt1 expression at nerve terminals is displayed, with the signal from transfected synapses normalized to untransfected synapses in the same field of view ± SEM (n = 12 mCer, n = 14 WT, n = 14 R383Q cells from three independent preparations, one-way ANOVA, ***p = 0.0002, **p = 0.007, ns = not significant).