Figure 8.

FM labeling of the posterior macula in single and double tmc1/2a/2b mutants (5 dpf). Otocysts of Tg(myo6b:β-Actin-mEGFP-pA) larvae of each genotype were injected with FM 4-64 vital dye. Representative top down views of the posterior macula are shown. GFP-positive hair bundles (green) were overlaid with FM 4-64 label (magenta) in the merge panels. tmc2a/2b double mutants show the strongest reduction in FM 4-64 label throughout the neuroepithelium, with the exception of a subset of hair cells at the periphery of the dorsal posterior region. We examined multiple specimens per genotype (WT, n = 8; tmc1, n = 8; tmc2a, n = 7; tmc2b, n = 5; tmc1/2a, n = 7; tmc1/2b, n = 5; tmc2a/2b, n = 5). Scale bar, 10 μm.