a, Protocol of Experiment 4a. Whole-brain concentration of (b) dopamine, (c) noradrenaline, (d) 5-HT, (e) 5-HIAA, and (f) the ratio of 5-HIAA and 5-HT concentrations as an indicator of 5-HT turnover of postmetamorphic zebrafish 2 weeks following chronic social isolation and/or acute buspirone treatment. *Significant difference from socially reared vehicle-treated group. Black # indicates significant difference from socially isolated vehicle-treated group. Orange # indicates marginal significance (p = 0.050). ns., No significant. veh: vehicle, bus: buspirone.