Figure 2.

Idebenone-stimulated astrocytic respiration is coupled to ATP synthesis. A, OCR measurements from primary rat cortical astrocytes. Idebenone (Ideb) was added at 0 (vehicle) to 80 μm, as indicated, followed by oligomycin (Oligo; 0.3 μg/ml). Data are the mean ± SE from three independent experiments. B, Bar graph representation of the data in A, showing the OCR values after oligomycin addition (gray bars) across increasing concentrations of idebenone. The OCR values just before oligomycin addition (“without oligomycin,” black bars) are plotted for comparison. There were no significant differences in OCR after oligomycin addition (gray bars, one-way ANOVA).