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. Author manuscript; available in PMC: 2020 Oct 27.
Published in final edited form as: Nat Methods. 2020 Apr 27;17(6):587–593. doi: 10.1038/s41592-020-0818-8

Figure 3|. Effects of LatB-induced actin disassembly on cell deformability.

Figure 3|

(a) LatB causes dose-dependent disassembly of actin cytoskeleton by scavenging actin monomers. (b–d) Deformability changes of HL60 cells upon LatB treatment observed in exemplary cDC (b), sDC (c), and xDC (d) experiments. 50%-density contour plots of deformability, D, versus cell diameter for HL60 cells treated with increasing dose of LatB are accompanied by deformability and cell diameter histograms. (e) Relative deformability, RD, as a function of LatB dose for cDC (purple), sDC (green) and xDC (yellow) measurements (closed circles). Data points represent means of medians of multiple experiment replicates (n = 3, 5, and 4, for cDC, sDC, and xDC, respectively), and error bars represent standard deviation. Lines are four-parameter log-logistic fits, with LatB half maximal effective concentration, EC50, indicated with open circles.