Table 2:
Glycan findings on ESI-QTOF before and after treatment
| Glycan structure | Before galactose | After galactose | P-value |
|---|---|---|---|
| 13C6-Neu5Ac2Hex5HexNac4 (IS) | 116553.33 ± 57108.29 | 142085 ± 55888.93 | N.S. |
| Hex5HexNAc2 (Man 5) | 3.13 ± 0.72 | 2.83 ± 0.74 | N.S. |
| Hex3HexNAc4 (Asialo, agalacto, biantennary) (A-gal) | 3.81 ± 6 | 3.18 ± 5.04 | N.S. |
| Hex6HexNAc2 (Man 6) | 3 ± 0.66 | 2.72 ± 0.59 | N.S. |
| Fuc1Hex3HexNAc4 (Asialo, agalacto, biantennary fucosylated) | 6.99 ± 2.28 | 6.67 ± 2.11 | N.S. |
| Hex4HexNAc4 (Monogalactosylated, asialo, biantennary) | 1.25 ± 0.81 | 1.06 ± 0.6 | N.S. |
| Neu5Ac1Fuc1Hex3HexNAc3 (Fucosylated Mono-antennary glycan) | 0.12 ± 0.04 | 0.09 ± 0.04 | 0.027 |
| Hex7HexNAc2 (Man 7) | 0.98 ± 0.29 | 0.83 ± 0.16 | N.S. |
| Neu5Ac1Hex4HexNAc3 (Monosialo,galactosylated,mono N-acetyl glucosamine biantennary) (M-GlcNAc) | 3.15 ± 1.04 | 3.04 ± 0.99 | N.S. |
| Hex8HexNAc2 (Man8) | 1.09 ± 0.35 | 0.94 ± 0.17 | N.S. |
| Neu5Ac1Hex4HexNAc4 (Monosialo, monogalactosylated, mono N-acetylglucosamine biantennary)(M-gal) | 7.34 ± 7.99 | 6.83 ± 7.96 | 0.011 |
| Fuc1Hex5HexNAc4 | 5.01 ± 1.8 | 4.5 ± 1.23 | N.S. |
| Hex9HexNAc2 (Man 9) | 0.85 ± 0.27 | 0.76 ± 0.17 | N.S. |
| Neu5Ac1Fuc1Hex4HexNAc4 (Monogalactosylated fucosylated biantennary) | 1.88 ± 0.4 | 1.83 ± 0.55 | N.S. |
| Neu5Ac1Hex5HexNAc4 (monosialo, biantennary) (M-sialo) | 11.44 ± 4.07 | 11.09 ± 3.16 | N.S. |
| Neu5Ac2Hex5HexNAc4 (Disialo biantennary) (Di-sialo) | 24.01 ± 9.68 | 27.91 ± 10.23 | 0.049 |
| Neu5Ac2Hex6HexNAc5 (Disialo triantennary) | 0.51 ± 0.26 | 0.61 ± 0.31 | N.S. |
| Neu5Ac2Fuc1Hex6HexNAc5 (Disialo triantennary fucosylated) | 0.2 ± 0.11 | 0.22 ± 0.11 | N.S. |
| Neu5Ac3Hex6HexNAc5 (Trisialo triantennary) (Tri-sialo) | 0.78 ± 0.36 | 1.09 ± 0.58 | 0.042 |
| Neu5Ac3Fuc1Hex6HexNAc5 (Trisialo triantennary fucosylated) | 0.24 ± 0.15 | 0.3 ± 0.15 | N.S. |
| NeuAc3Hex7HexNAc6 (Trisialo tetra-antennary) | 0.06 ± 0.06 | 0.07 ± 0.07 | N.S. |
| Total Glycan Signal | 3004.08 ± 653.6 | 2478.09 ± 323.51 | N.S. |
| Selected ratios | |||
| Man5/9 | 4.11 ± 1.49 | 3.98 ± 1.82 | N.S. |
| Calculated Di-sialo Concentration | 0.48 ± 0.43 | 0.44 ± 0.31 | N.S. |
| M-gal/Di-SA | 0.74 ± 1.27 | 0.45 ± 0.68 | 0.011 |
| M-sial/di-sial | 0.51 ± 0.1 | 0.42 ± 0.09 | 0.017 |