Figure 7.

Effects of potential HIF-1α inhibitors on nickel-induced HIF-1α and miR210 upregulation. Several compounds that reportedly inhibited HIF-1α were tested with the indicated concentrations and the same treatment procedure as melatonin. The effects of 2-deoxyglucose (2-DG), α-ketoglutarate (α-KG) derivative, N-acetylcysteine (NAC), and rapamycin (Rapa) on nickel-induced HIF-1α accumulation were measured by western blot (a and b). The effects of HIF-1α inhibitors on nickel-induced miR210 increase were measured by qRT-PCR assay (c and d). The error bar reflects the S.E.M. of at least three independent experiments. ^∗P < 0.05 compared with the nickel-free control group, and ^#P < 0.05 compared with the same nickel concentration but with the melatonin- or NAC-free group.