Table 1.

PCR conditions of examined genes in mice.

Gene	Product size (bp)	Annealing temperature	Primer sequence
BAX	483	53°C, 1 min	Forward 5′-acacctgagctgaccttggagca-3′ Reverse 5′-agacacagtccaaggcagtggga-3′
Caspase-3	478	53°C, 1 min	Forward 5′-acgcagccaacctcagagagaca-3′ Reverse 5′-ttgtgcgcgtacagcttcagcat-3′
XIAP	522	59°C, 1 min	Forward 5′-gggtgggtcttagaggggcttat-3′ Reverse 5′-acaagccatatacaccaggcgtc-3′
Bcl-XL	371	54°C, 1 min	Forward 5′-ccactggccacagcagcagttt-3′ Reverse 5′-aaaagtgtcccagccgccgtt-3′
SOD	320	55°C, 1 min	Forward 5′-gtcctttcctgcggcgcctt-3′ Reverse 5′-cgccgggccaccatgtttct-3′
Catalase	500	58°C, 1 min	Forward 5′-ggcccctcctcgttcaggatgt-3′ Reverse 5′-gccattcatgtgccggtgacca-3′
HO-1	460	55°C, 1 min	Forward 5′-tcaggtgtccagagaaggcttta-3′ Reverse 5′-aggtgtcatctccagagtgttca-3′
NLRP3	434	56°C, 1 min	Forward 5′-agctgctggcctgacccaaa-3′ Reverse 5′-agccccgtgcacacaatcc-3′
TNF-α	346	56°C, 1 min	Forward 5′-aactagtggtgccagccgat-3′ Reverse 5′-cttcacagagcaatgactcc-3′
IL-1-β	409	58.5°C, 1 min	Forward 5′-ccgtggaccttccaggatga-3′ Reverse 5′-gatccacactctccagctgc-3′
IL-10	515	59°C, 1 min	Forward 5′-agagcaaggcagtggagcaggt-3′ Reverse 5′-aagggccctgcagctctcaagt-3′
INF-γ	401	58.5°C, 1 min	Forward 5′-cccacaggtccagcgccaag-3′ Reverse 5′-gctgtcccccacccccagat-3′
β-actin	560	56°C, 1 min	Forward 5′-agatccacaacggatacatt-3′ Reverse 5′-tccctcaagattgtcagcaa-3′

PCR cycle of respective genes are shown, while temperature and time of denaturation and elongation steps of each PCR cycle are 94°C, 30 s and 72°C, 60 s, respectively; annealing temperature was indicated for each gene in the table.