Table 1.
Validated OpenData portal assays and those under development.
| Assay Name | Assay Type | Target Category | Detection Strategy | Cell Line | Status |
|---|---|---|---|---|---|
| Spike-ACE2 protein-protein interaction (AlphaLISA) | Proximity | Viral Entry | AlphaLISA | n/a | Screening in progress |
| Spike-ACE2 protein-protein interaction (TruHit counterscreen) | Proximity | Counterscreen | AlphaLISA | n/a | Screening in progress |
| Spike-ACE2 protein-protein interaction (QD) | Proximity | Viral Entry | High-content imaging | n/a | Under development |
| Spike-ACE2 binding | Biophysical | Viral Entry | Bio-Layer Interferometry | n/a | Screening in progress |
| ACE2 binding | Biophysical | Viral Entry | Microscale thermophoresis | n/a | Screening in progress |
| ACE2 enzymatic activity | Biochemical | Viral Entry | Fluorescence | n/a | Screening in progress |
| ACE2 internalization | Cell-based | Viral Entry | High-content imaging | HEK293 | Under development |
| TMPRSS2 enzymatic activity | Biochemical | Viral Entry | Fluorescence | n/a | Under development |
| 3CL enzymatic activity | Biochemical | Viral replication | Fluorescence | n/a | Under development |
| RdRp enzymatic activity | Biochemical | Viral replication | Fluorescence | n/a | Under development |
| SARS-CoV-2 replicon | Cell-based | Viral replication | Luminescence | TBD | Under development |
| Pseudotyped particle entry | Cell-based | In vitro infectivity | Luminescence | TBD | Under development |
| Virus-like particle entry | Cell-based | In vitro infectivity | TBD | TBD | Under development |
| SARS-CoV-2 cytopathic effect (CPE) | Cell viability | Live virus infectivity | Luminescence | Vero E6 | Screening in progress |
| SARS-CoV-2 cytopathic effect (host toxicity counterscreen) | Cell viability | Counterscreen | Luminescence | Vero E6 | Screening in progress |
| SARS-CoV-2 Viral Infection | Cell-based | Live virus infectivity | TBD | TBD | Under development |
| SARS-CoV-2 Viral Infection (host toxicity counterscreen) | Cell-based | Counterscreen | TBD | TBD | Under development |
| Spike protein detection | Proximity | Live virus infectivity | AlphaLISA/HTRF | n/a | Under development |
| Nucleocapsid protein detection | Proximity | Live virus infectivity | AlphaLISA/HTRF | n/a | Under development |
QD, quantum dot; TBD, to be determined; HTRF, Homogeneous Time Resolved Fluorescence