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. 2020 Jun 2;13:4919–4929. doi: 10.2147/OTT.S238808

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© 2020 Jiang et al.

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PAX6 is a target of miR-142-5p in NSCLC cells. (A) The predicted binding sites between miR-142-5p and PAX6 were listed. (B–D) The interaction between miR-142-5p and XIST was confirmed using the dual-luciferase reporter assay (B) and RIP assay (C and D) in A549 and H1299 cells. A549 and H1299 cells were transfected with miR-NC, miR-142-5p, anti-miR-NC, or anti-miR-142-5p. (E–H) The mRNA and protein levels of PAX6 in transfected cells were detected using qRT-PCR (E and F) or Western blot (G and H), respectively. *P<0.05.