Figure 1. Rewilding Alters the Proportion of Lymphoid Cell Subsets.

(A) Representative flow cytometry plots and quantification of CD62L^hiCD44^lo, CD62L^hiCD44^hi and CD62L^loCD44^hi CD8⁺ T cells in the peripheral blood of rewilded mice (Wild) and control mice maintained in the laboratory condition (Lab). Cells were gated on Live⁺CD45⁺Myeloid⁻ CD3⁺CD19⁻CD8⁺. N = 79 lab and 101 rewilded mice (B) Quantification of the indicated CD8⁺ T cells in the mesenteric lymph nodes (MLNs). (C) Mean fluorescent intensity of MLN CD8⁺ T cells expressing activation marker KLRG1. (D) Representative images of small intestinal sections stained with H&E or anti-CD8 antibodies and quantification. N = 50 villi from 5 mice per condition. Mean and spread are shown. **** P < 0.0001 by two-tailed Student’s t-test between groups, (A) to (D). See also Figure S1.