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. Author manuscript; available in PMC: 2021 May 13.
Published in final edited form as: Cell Host Microbe. 2020 Mar 24;27(5):809–822.e6. doi: 10.1016/j.chom.2020.02.015

Figure 2. Rewilding Leads to an Expansion in Granulocytes.

Figure 2.

(A) Functional classification of 112 differentially regulated genes (log2FC>1.5) by Ingenuity pathway analysis comparing RNA-Seq of MLN cells from lab and rewilded mice. The arrowed line marks where the p-value becomes less than 0.05. N = 40 lab and 46 rewilded mice. (B) Representative forward scatter (FSC) and side scatter (SSC) flow cytometry plots and quantification of SSChi cells (granulocytes) and myeloid cells in the peripheral blood of lab and rewilded mice. All cells were gated on Live+CD45+ and myeloid cells were identified by cell surface markers CD11b/CD11c/DX5. N = 79 lab and 101 rewilded mice. (C) Quantification of the absolute number of MLN cells expressing neutrophil markers (CD11b+Ly6G+). Mean and spread are shown. **** P < 0.0001 by two-tailed Student’s t-test between groups, (B) to (C).