Figure 2.

Early B Cell Response to SARS-CoV-2 Is Diverse and Largely Unmutated

(A) Class switched (IgM⁻ IgG⁺) B cells were stained with SARS-CoV-2 S2P labeled with BV710 or PE.

(B) SARS-CoV-2 S2P⁺ IgG⁺ B cells were further analyzed for binding to Alexa Fluor 647-labeled SARS-CoV-2 RBD.

(C–E) Individual SARS-CoV-2 S2P⁺ IgG⁺ B cells were sorted into separate wells of a 96-well plate and sequenced using RT-PCR. VH (C), VK (D), and VL (E) gene usage of successfully sequenced S2P-specific B cells.

(F and G) CDRH3 (F) and CDRL3 (G) length distributions of successfully sequenced S2P-specific B cells.

(H) Number of amino acid substitutions from germline in S2P-specific heavy and light chains.

See also Figure S1 and Table S1.