Table 3.
Primers and probes used in this study for the qPCR assays.
| Primers and Probes | Oligo set | Sequence (5'-3') | Target gene | Target species | Source |
|---|---|---|---|---|---|
| Bc3 F | GCTGTAATTTCAATGTGCAGAATCC | ||||
| Bc3 R | Bc3 | GGAGCAACAATTAATCGCATTTC | IGS | B. cinerea | [30] |
| Bc3 Probea | TCACCTTGCAATGAGTGG | ||||
| ITS1 S Tharz | TACAACTCCCAAACCCAATGTGA | ||||
| ITS1 R Tharz | Tharz | CCGTTGTTGAAAGTTTTGATTCATTT | ITS | T. harzianum | [7] |
| ITS1 TM Tharz Probea | AACTCTTATTGTATACCCCCTCGCGGGT | ||||
| TviriF1 | CCCAAACCCAATGTGAACCA | T. atroviride | |||
| TviriR1 | Tviri | TCCGCGAGGGGACTACAG | ITS | T. koningii | EPA USA |
| TviriP1 Probea | CCAAACTGTTGCCTCGGCGGG | T. viride | |||
| TrichoBV1 F | TCAATCCCTGTGTGGTTCAG | ||||
| TrichoBV1 R | Tatrov | GAACCCACGCATACTTGAAA | tef1-α | T. atroviride | Present study |
| TrichoBV1 Probeb | CGGCTTCCTATTGATTGAAACA |
a
FAM (6-carboxy-fluorescein)-labelled probe at.3’.
b
VIC (trade name)-labelled probe at.5’.