. 2020 May;82(2):261–280. doi: 10.18999/nagjms.82.2.261

Supplementary Table 2.

Western blotting band intensities were measured according to the Materials and methods

Fig. 3 b
	K562			K562/ADR
	Control	RSV D1	VTC D1	Control	RSV D1	VTC D1
cleaved PARP	1	18.81±10.18 ^*	20.67±6.42 ^*	1	23.84±9.60 ^*	43.48±13.84 ^**
cleaved Caspase 3	1	8.96±3.86 ^*	9.61±3.67 ^*	1	6.24±4.52 ^NS	14.89±7.43 ^*
BCL-xL	1	0.85±0.06 ^NS	0.35±0.13 ^***	1	0.82±0.35 ^NS	0.30±0.10 ^*
Bak	1	5.29±6.34 ^NS	62.80±15.98 ^***	1	6.38±3.74 ^NS	51.48±20.06 ^**

Fig. 5 c
	K562			K562/ADR
	Control	VTC D1	VTC D2	Control	VTC D1	VTC D2
NSMase 2	1	0.57±0.48 ^NS	1.25±1.06 ^NS	1	0.43±0.18 ^*	0.65±0.34 ^NS
ACDase α	1	0.72±0.03 ^NS	0.94±0.78 ^NS	1	0.74±0.59 ^NS	1.02±0.88 ^NS
ACDase β	1	0.79±0.24 ^NS	0.86±0.12 ^NS	1	0.80±0.38 ^NS	0.62±0.03 ^NS
SPHK1	1	0.20±0.05 ^***	0.18±0.13 ^***	1	0.29±0.11 ^***	0.16±0.07 ^***
SPHK2	1	0.34±0.20 ^**	0.14±0.12 ^***	1	0.19±0.30 ^**	0.14±0.24 ^**
GCS	1	0.05±0.06 ^***	0.17±0.29 ^**	1	0.37±0.33 ^*	0.09±0.10 ^**
SPL	1	0.06±0.05 ^***	0.02±0.03 ^***	1	0.05±0.05 ^***	0.01±0.01 ^***
DES1	1	1.07±0.47 ^NS	0.98±0.49 ^NS	1	0.92±0.09 ^NS	0.71±0.17 ^*
CERS2	1	0.15±0.18 ^***	0.01±0.02 ^***	1	0.82±0.43 ^NS	0.28±0.37 ^NS
CERS3	1	0.27±0.05 ^***	0.16±0.11 ^***	1	0.16±0.27 ^**	0.12±0.21 ^**

Fig. 6 e
	K562
	Control	SKI	ABC	PDMP	SKI+PDMP	ABC+PDMP
BCL-xL	1	0.51±0.13 ^*	0.94±0.11 ^NS	0.74±0.22 ^NS	0.76±0.22 ^NS	0.78±0.27 ^NS
MCL1	1	0.13±0.17 ^**	0.26±0.32 ^*	0.48±0.38 ^NS	0.23±0.29 ^*	0.21±0.13 ^**

Fig. 6 f
	K562
	Control	EGCG	Quercetin	Curcumin	Sulforaphane	Adriamycin
SPHK1	1	0.08±0.12 ^*	0.50±0.34 ^NS	0.63±0.15 ^NS	0.85±0.66 ^NS	0.46±0.14 ^**
SPHK2	1	0.04±0.02 ^***	0.37±0.10 ^**	0.29±0.17 ^**	0.40±0.35 ^**	0.20±0.09 ^***
GCS	1	0 ^***	0.17±0.09 ^***	0.16±0.08 ^***	0.28±0.07 ^***	0.15±0.07 ^***

Band intensity of control K562 or K562/ADR cells was regarded as 1.0, respectively. The mean ± SD was shown and the statistical significances were evaluated between control cells and treated cells using one-way factorial analysis of variance and the multiple comparison test (Dunnet method). Shaded area indicates the statistical significance compared with control cells.

* p<0.01, ** p<0.001, *** p<0.0001, NS: not significant