Fig. 4.
DGAT2 promotes HFD-induced peritoneal dissemination and lung metastasis in vivo. Female nude or SCID mice fed the ctrl diet or HFD were injected intraperitoneally with 3 × 106 scrambled vector or DGAT2 shRNA transduced cells and were sacrificed after 30 days. (A) Representative intestines and H&E staining of scattered tumors in nude mice (N = 6). (B) The quantification of metastases in nude mice was counted. (C–D) Representative intestines and H&E staining of scattered tumors in SCID mice (N = 6). The quantification of metastases in SCID mice was counted. (E) Representative results of H&E staining of metastatic lung nodules from nude mice injected with DGAT2 knockdown and control cells via the tail vein for 30 days (scale bars = 200 μm). (F) Metastatic nodules under the microscope were counted and recorded. **P < 0.01, Student's t-test, mean ± SD.