Table 1.

Sequence primers for detection of the reverse transcription products and HIV-1 mRNA.

Targets	Primer sequences	References
Early product (R5/U5)	Early F:5′-GCTCTCTGGCTAACTAGGGAAC-3′ Early R: 5′-TGACTAAAAGGGTCTGAGGGAT-3′	Heredia et al. (2015)
Late product (U5/PBS/Ψ)	MH531 F: 5′-TGTGTGCCCGTCTGTTGTGT-3′ MH532 R 5′-GAGTCCTGCGTCGAGAGATC-3′	Mbisa et al. (2009)
2-LTR circles	MH535 F:5′-AACTAGGGAACCCACTGCTTAAG-3′ MH536 R: 5′-TCCACAGATCAAGGATATCTTGTC-3′	Mbisa et al. (2009)
Integrated provirus	Alu-based nested PCR First round PCR: Alu F:5′GCCTCCCAAAGTGCTGGGATTACAG-3′ Gag R: 5′-GCTCTCGCACCCATCTCTCTCC-3′ Second round PCR: LTR-R-F: 5′-GCCTCAATAAAGCTTGCCTTGA-3′ LTR-U5-R: 5′-TCCACACTGACTAAAAGGGTCTGA-3′	Heredia et al. (2015)
Cellular HIV mRNA (Full length)	US.1a -F: 5′-GCTTGCTGAAGCGCGCACGG-3′ US.2a-R: 5′-CGTTCTAGCTCCCTGCTTGC-3′	Hermankova et al. (2003)