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Mucus production measured with the ratio of blue (RGBB ratio) at control (ΔP=0 cmH2O) and ΔP=5, 10, 15 and 20 cmH2O continuous positive airway pressure for 8 h of Calu-3 and RPMI 2650 cells. Data are presented as mean±sd, n=3. ****: p<0.0001.