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Tissue preparation for mechanical testing and tissue-level experiments. (a) Illustration of tissue explant with radial (R) and circumferential (C) directions used for mechanical testing (region 1) and that used for histology (region 2). (b) Illustration of the biaxial mechanical testing apparatus, with four centrally located fiducial markers, sutures mounting the tissue sample, and displacements xR and xC in the radial and circumferential directions, respectively.
