Table 4.

(1→3)-β-D-glucan values for patients with microbiologically confirmed PCP and possible PCP

PCP	108 patients	(1→3)-β-D-glucan (pg/mL) ± SD	qPCR Ct ± SD
Possible PCP	66 (61%)	256.06 ± 183.48	35.01 ± 3.8
Microbiologically confirmed PCP (DIF and qPCR positive)	42 (39%)	467.5 ± 95.5	27.2 ± 4.6
Subgroup definite PCP (DIF positive and qPCR Ct < 30 and response to treatment)	26 (24%)	494.81 ± 17.73*
P value		< 0.001	< 0.001

Ct = threshold cycle; DIF = direct immunofluorescence; PCP = pneumocystis pneumonia; qPCR = real time PCR; SD = standard deviation

* Area under the curve (AUC) to predict PCP (positive DIF and qPCR) with measurement of (1→3)-β-D-glucan: AUC = 0.817, 95% CI: 0.736–0.898, sensitivity = 83.3%, specificity = 64.6%, cut off 496.45 pg/mL, p < 0.001