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Genotype identification of offspring. (A) The mutations in F0 generation were detected by T7E I digestion using PCR products amplified from F0 rat tail genomic DNA by Primer. T7E I−, before T7E I digestion. T7E I+, after T7E I digestion. "
", mutation band. WT, wild type as negative control. M, DNA molecular weight marker. (B) Gene sequence near the target in WT and Slco1b2 KO rats. "…", missing sequence.
