Table I. Primers used in the study.

Gene	Primer Sequence	Purpose	Product Size (bp)
Nhr38-BSP-F	5′-TTTTGAGGTATTTATAAAAAGTTTGG-3′	Identification of methylation level	396
Nhr38-BSP-R	5′-CTTAAACACTTAAACCCTCTCTAAA-3′
Pcdh10-BSP-F	5′-TTTTTTTTGTAATAATATTGTTGTTG-3′	Identification of methylation level	321
Pcdh10-BSP-R	5′-TCCCTATCAAAAAATTACAAAC-3′
Ccdc158-BSP-F	5′-TTTATAGTTTTGGGGTTTAGAGG-3′	Identification of methylation level	428
Ccdc158-BSP-R	5′-ACAAATTCTAACTTTCACATCCA-3′
Arnt2-BSP-F	5′-TTTGATATAGAGGGGGTTATAAGT-3′	Identification of methylation level	264
Arnt2-BSP-R	5′-AAAATAACTTTTTATAACCCAATTTAT-3′
Nhr38- F	5′-AGGCCTTCAAGCTCGATGAT-3′	qRT-PCR	147
Nhr38-R	5′-AGCTTGGTGTTGGAGGAGAA-3′
Pcdh10-F	5′-TCACGATGACCTCAGACACC-3′	qRT-PCR	136
Pcdh10-R	5′-AGTCAGTTCCAACCGGCTAA-3′
Ccdc 158-F	5′-GTTCTGGAGAGATTTGGCGG-3′	qRT-PCR	145
Ccdc 158-R	5′-CCAGACCAAAGACCAAAGGC-3′
Arnt2-F	5′-ACCAGGCTATCTAGTGACGC-3′	qRT-PCR	140
Arnt2-R	5′-GGGCTGTTCTTAGTTCCCCT-3′
Actin-F	5′-TGGTGGGTATGGGTCAGAAAG-3′	qRT-PCR	217
Actin-R	5′-CTGTTGGCTTTGGGGTTCA-3′

qRT-PCR, quantitative RT-PCR.