Figure 5.

TRAF2 was involved in the mediation of AP-1 transcription activity. (a) Cyclin D1 mRNA was in TRAF2 knockdown CNE-1(left) and HK-1 (right) cells. (b) TRAF2 knockdown inhibited AP-1 transcriptional activity. AP-1 luciferase reporter was transfected into TRAF2-deficient CNE1 cells, and the activity of luciferase was determined as described. ^∗∗∗p < 0.001 vs. control. (c, d) TRAF2 mediated the activities of transcription factors. TRAF2-deficient CNE1 or HK-1 cells (c) or TRAF2-overexpression NP460 cells (d) were starved overnight and then stimulated with EGF at different time points; cell lysates were collected subjected to western blotting, and the expression of the indicated protein was probed with the corresponding antibody.