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. 2020 May 7;10(5):729. doi: 10.3390/biom10050729

Figure 4.

Figure 4

Synthetic lethal interaction between pus1 and sup70-33/sup70-65 was suppressed by met22 mutation. (A) Plasmid shuffle approach (as in Figure 1) for analysis of sup70 complementation by SUP70 or sup70-65 in absence of PUS1 (pus1∆), MET22 (met22∆), or both. All strains carried the [SUP70-URA3] plasmid. Indicated strains additionally carried HIS3-plasmids (s.c. vector HIS3) providing SUP70 or sup70-65 as indicated. Top: wild-type BY4741. (B) Plasmid shuffle approach for analysis of sup70 complementation by SUP70 or sup70-33 in absence of PUS1 (pus1∆), MET22 (met22∆), or both.