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Detection of different subpopulations of Pf-derived EVs using the ZE5 analyzer. (A) Top row: Mixed population of Pf-derived EVs (ring and trophozoite) stained with HO. Gating was done according to the unstained EVs. Bottom row: medium-derived ‘EVs’ were used as a control for absence of DNA content. (B) Pf-derived EVs from different parasite stages (ring and trophozoite) were stained with HO to visualize the DNA inside the EVs. Gating was done according to the unstained EV population.
