Table 5.

IL-10 polymorphism in the three groups in correlation with other gene expression. IL-10 severe SNPs (ATA/ATA-ATA/ACC-ACC/ACC) included VDRs SNPs, especially seen in Taql, Apal, and Fokl (p < 0.05) (red and yellow with Taql TT/Tt, Apal AA/Aa, Foql FF/Ff); IL-10 moderate SNPs (ACC/GCC) included VDRs SNPs seen in VDR Taql, 17 patients TT (red) (p < 0.05) and 20 patients Tt (yellow). The SNPs in IL-10 functionally expressed by ATA/ATA-ATA/ACC-ACC/ACC genotypes have no statistically significant relation with COLIA1 gene expression. IL-10 depletion had very low effect on COLIA1 gene expression, genotypes analysis revealed 2 ss (red color), 9 Ss (yellow color), and 19 SS (green color); SNPs in IL-10 functionally expressed by ATA/ATA-ATA/ACC-ACC/ACC genotypes showed a certain degree of correlation with ILs-1 group. Patients with IL-10 depletion showed a substantial affection IL-1gene expression, genotypes analysis showed 10 severe (red color), 17 moderate (yellow color), and seven without alteration (green color). IL-10 polymorphisms (ATA/ATA-ATA/ACC-ACC/ACC) and TNF-α showed a moderate inverted type of correlation. SNPs at IL-10 showed a quite substantial effect on patient’s TNF-α gene expression. Severe IL-10 depletion showed no effect on TNF-α gene activity that was statistically significant (p > 0.05). From severe to moderate to normal IL-10 gene expression TNF-α was shown to be always substantially regularly expressed from 65% to 78% to 77% in the GG haplotype, 32%, 23%, and 23% in the AG haplotype and practically 0–3% in AA haplotype (red= severity grade 2; yellow = severity grade 1; green = severity grade 0, normal expression).

Gene Expression	Count (Subjects):	IL-10
		22	40	34
		GCC/GCC	ATA/GCC	All Severity = 2
	Grade of SNPs and Haplotype	0	1	2
IL-1 α/β/RN	0	32% = 7	35% = 14p	21% = 7p
	1	55% = 12p	35% = 14p	50% = 17p
	2	14% = 3p	30% = 12p	29% = 10p
TNF-α	0 GG	77% = 17p	78% = 31p	65% = 22p
	1 AG	23% = 5p	23% = 9p	32% = 11p
	2 AA	0% = 0p	0% = 0p	3% = 1p
Tagl VDR	0 tt	5% = 1p	8% = 3p	18% = 6p
	1 Tt	59% = 13p	50% = 20p	41% = 14p
	2 TT	36% = 8p	43% = 17p	41% = 14p
Apal VDR	0 aa	14% = 3p	25% = 10p	6% = 2p
	1 Aa	55% = 12p	45% = 18p	71% = 24p
	2 AA	32% = 7p	30% = 12p	24% = 8p
Bsml VDR	0 bb	32% = 7p	50% = 20p	44% = 15p
	1Bb	23% = 15p	45% = 18p	50% = 18p
	2 BB	45% = 0p	5% = 2p	6% = 2p
Fokl VDR	0 ff	9% = 2p	13% = 5p	3% = 1p
	1 Ff	50% = 11p	55% = 22p	50% = 17p
	2 FF	41% = 9p	33% = 13p	47% = 16p
COLIA 1	0 SS	32% = 10p	35% = 15p	21% = 19p
	1 Ss	55% = 9p	35% = 14p	50% = 9p
	2ss	14% = 3p	30% = 1p	29% = 2p