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. 2020 Apr 30;10(5):695. doi: 10.3390/biom10050695

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Myostatin administration on C2C12 cells increased inflammatory signaling in C2C12 myoblasts, whereas in myotubes the effect was very small. (a) Phosphorylated STAT3^Tyr705 and total STAT3 in myoblasts and myotubes. (b) Phosphorylated C/EBPβ^Thr235 and total C/EBPβ in myoblasts and myotubes. In the figures, the values are presented as normalized to CTRL = 1. (c) Nontreated CTRL myoblasts and myotubes of the 2-h and 24-h time-points were pooled, and the values are presented as normalized to myoblasts = 1. (d) Representative blots. In A–B, N = 6 per group. In C, N = 12 per group. *, **, and *** = p < 0.05, p < 0.01, and p < 0.001, respectively. CTRL (-) = control group, MSTN (+) = myostatin group.