Table 3.
In vitro mortality percentages and lethal concentration (95%) attributed to the effect of four Prosopis laevigata sub-fractions and compounds derived from EtAc-F against Haemonchus contortus infective larvae (L3).
| Sub-Fractions and Compound (Step 2) |
Mean of Recovered Larvae Dead Larvae/Total |
% Mortality ±SD | Lethal Concentrations 95% (CI Limits Lower-Upper) |
|
|---|---|---|---|---|
| C1F1 (mg/mL) | LC50 (mg/mL) | LC90 (mg/mL) | ||
| 15 | 67.5/84.75 | 79.47 b | ||
| 7.5 | 18.15/76.75 | 24.12 d | ||
| 3.7 | 8/77.2 | 10.47 d | ||
| C1F2 (mg/mL) | ||||
| 15 | 11.2/70 | 24.45 ± 2.44 d | ||
| C1F3 (mg/mL) | ||||
| 15 | 4.5/66.25 | 6.82 ± 0.72 e | ||
| C1F4 (mg/mL) | ||||
| 15 | 3/78.5 | 5.75 ± 2.56 e | ||
| C2F1 (mg/mL) | ||||
| 15 | 127.5/0 | 100 a | 2.82 (2.72–2.92) |
3.82 (3.69–3.96) |
| 7.5 | 126.5/0 | 100 a | ||
| 3.75 | 107.5/122.5 | 87.8 ± 3.46 b | ||
| 1.87 | 2/120 | 2.27 ± 0.45 e | ||
| C2F2 (mg/mL) | ||||
| 15 | 120.0/0 | 100 a | 2.68 (2.45–2.86) |
3.84 (3.67–4.02) |
| 7.5 | 121/0 | 100 a | ||
| 3.7 | 101.5/115 | 88.2 ± 0.47 b | ||
| 1.8 | 10.75/107.5 | 10.0 ± 0.77 d | ||
| C2F3 (mg/mL) | ||||
| 15 | 117/0 | 100 a | 3.04 (2.89–3.19) |
5.66 (5.32–6.07) |
| 7.5 | 109.7/116.5 | 94.22 ± 1.63 b | ||
| 3.7 | 77.7/115.2 | 67.47 ± 1.29 c | ||
| 1.8 | 2.5/115.2 | 2.15 ± 0.51 e | ||
| Isorhamentin (mg/mL) (1) | ||||
| 15 | 126/0 | 100 a | 2.07 (1.98–2.16) |
3.79 (3.60–4.02) |
| 7.5 | 127/0 | 100 a | ||
| 3.7 | 101.5/115 | 88.25 ± 0.47 b | ||
| 2.5 | 73.25/107.5 | 68.15 ± 4.46 c | ||
| 1.2 | 14.5/106.75 | 13.6 ± 3.19 d | ||
| 0.6 | 2.25/105.75 | 2.15 ± 0.50 e | ||
| Isorhanmetin and luteolin (2.5 mg/mL) (1,2) |
0/103.82 | 0 f | ||
| Luteolin (2.5 mg/mL) (2) | 0.5/107.2 | 0.47 ± 0.95 f | ||
| 4′-O-methylcatechin (2.5 mg/mL) (3) | 0/104.2 | 0 f | ||
| Distilled water | 0/71.25 | 0 f | ||
| MeOH (4%) | 0/69.87 | 0 f | ||
| Ivermectin (0.5 mg/mL) | 73.5/73.5 | 100 a | ||
| Variation coefficient | 3.56 | |||
| R2 | 0.99 | |||
Note: Means inside same column with different letter indicate significant differences (Tukey test) p < 0.05; MeOH = Methanol, SD = Standard deviation; CI = Confidence Interval.