Table 2.
Analytical methods specifications.
| Drug | Detector | Column | Analysis | Mobile Phase | Flow Rate, mL/min | λ, nm | LOQ 1, μg/mL | RT, min |
|---|---|---|---|---|---|---|---|---|
| VA | UV | Kinetex Phenomenex® C18 100 Å (5 μm; 150 × 4.6 mm) | Isocratic | NaH2PO4/Na2HPO4 (40:60, pH = 6.95, 50 mM) | 1.0 | 210 | 0.025 | 5.0 |
| hLF 1-11 | DAD | Phenomenex® Aeris widepore XB-C8 200Å (3.6 μm; 150 × 4.6 mm) | Linear gradientfrom 5% to 70% solvent A over 25 min | Solvent A (0.05% TFA in acetonitrile); Solvent B (0.05% of TFA in water) | 1.0 | 220 | 1.0 | 5.4 |
1 LOQ: limit of quantitation; RT: retention time.