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. 2020 May 22;8(5):131. doi: 10.3390/biomedicines8050131

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) AR-V7 and (B) normalized AR-V7 copies/mL, in responder (R) and non-responder (NR) samples detected with the IA-RNA protocol, the generic RNA protocol and the PA-RNA protocol. The IA-RNA protocol distinguished between R and NR patients better than the other protocols on the basis of normalized AR-V7 results. (C) The average AR-V7 allelic frequency detected after IA-based enrichment and generic extraction, evaluated as: AR-V7 copies/WT copies + AR-V7 copies). (D) The difference between the NR and the R patients for the two isolation methods.