Figure 4.

In vivo effects of Sk-EE on HCl/EtOH-induced gastritis mice. (a) The indicated doses of Sk-EE and control compound ranitidine were orally injected into mice three times in two days. HCl/EtOH was orally administered to mice one hour before sacrifice. The stomachs of sacrificed mice were isolated and inflammatory lesions were photographed. (b) Inflammatory lesion areas of each group were numerically analyzed by ImageJ (National Institutes of Health, Bethesda, MD, USA) and graphed. (c) The stomach samples of the gastritis mouse model were ground and lysed with buffer. Protein levels of total and phosphorylated forms of IκBα and control protein β-actin were analyzed by western blotting. # p < 0.05 and ## p < 0.01 compared to normal group; * p < 0.05 and ** p < 0.01 compared to control group. Data presented (b) are expressed as mean ± SD of experiments performed with 3 samples. +: treatment, −: no treatment.