Table 1.
Characteristics of antibodies (host, concentration, and exposure time).
| Antibody | Host | Dilution | Exposure (Time/Temperature) |
|---|---|---|---|
| p65 | Rabbit | 1:2500 | Overnight/4 °C |
| p-p65 | Rabbit | 1:2500 | Overnight/4 °C |
| p50 | Rabbit | 1:2500 | Overnight/4 °C |
| p-p50 | Rabbit | 1:2500 | Overnight/4 °C |
| Lamin A/C | Mouse | 1:2500 | Overnight/4 °C |
| p85 | Rabbit | 1:2500 | Overnight/4 °C |
| IKKα | Rabbit | 1:2500 | 2 h/RT |
| p-IKKα/β | Rabbit | 1:2500 | Overnight/4 °C |
| IκBα | Rabbit | 1:2500 | Overnight/4 °C |
| p-IκBα | Mouse | 1:2500 | 2 h/RT |
| Syk | Rabbit | 1:2500 | Overnight/4 °C |
| p-Syk | Rabbit | 1:2500 | Overnight/4 °C |
| Src | Rabbit | 1:2500 | Overnight/4 °C |
| p-Src | Rabbit | 1:2500 | 2 h/RT |
| p85 | Rabbit | 1:2500 | 2 h/RT |
| β-actin | Rabbit | 1:2500 | 2 h/RT |
| HA | Mouse | 1:2500 | 2 h/RT |
| Myc | Mouse | 1:2500 | 2 h/RT |
| Rabbit IgG | Goat | 1:2500 | 2 h/RT |
| Mouse IgG | Horse | 1:2500 | 2 h/RT |
RT: room temperature.