Table 2.
Instrument and working conditions for high-performance liquid chromatography (HPLC) of quercetin, kaempherol, silibinin, and genistein.
| Instrument | Condition A | Condition B | ||||
|---|---|---|---|---|---|---|
| Column | CAPCELL PAK C18 MG, 4.6 mm I.D. × 250 mm | |||||
| Detector | UV-Vis Detector | |||||
| Wavelength | 254 nm | 350 nm | ||||
| Analyzed period | 30 min | 40 min | ||||
| Solvent | Solvent A | 2% acetic acid in water | Solvent A | 0.1% formin acid in MeOH:water = 10:90 | ||
| Solvent B | 0.5% acetic acid in water:ACN = 50:50 | Solvent B | 0.1% formin acid in MeOH:water = 90:10 | |||
| Flow rate | 1 mL/min | 0.4 mL/min | ||||
| Volume | 10 µL | 10 µL | ||||
| Gradient | Time (min) | Composition (%) | Time (min) | Composition (%) | ||
| A | B | A | B | |||
| 0 | 28 | 72 | 0 | 40 | 60 | |
| 20 | 0 | 100 | 20 | 40 | 60 | |
| 30 | 0 | 100 | 25 | 70 | 30 | |
| - | - | - | 40 | 70 | 30 | |
Condition A: quercetin, kaempherol. Condition B: silibinin, genistein.