Figure 6.

The effect of necrotic condition or nutritional deficiency on the mRNA expression of SCD (a,b) and SCD5 (c,d). Human brain (glioblastoma astrocytoma) U-87 MG cell line cultured in eagle’s minimum essential medium (EMEM) with 10% fetal bovine serum (FBS), 2 mM l-glutamine, 1 mM sodium pyruvate, 1% non essential amino acids, 100 U/mL penicillin, and 100 µg/mL streptomycin. U-87 MG cells were seeded in 6-well plates. After 72 h incubation, cells were washed three times with phosphate-buffered saline (PBS) solution and cultured for 24 h under three different conditions (control, nutrient deficiency, and necrotic). Control cells were suspended in full medium, starved cells were grown in medium with a low concentration of l-glutamine (0.2 mM) and without sodium pyruvate. For the induction of necrotic conditions, cells were incubated in medium supplemented with 200 µM H₂O₂. After 24 h of incubation, the cells were trypsinized and analyzed using qRT-PCR. **—statistically significant difference in the expression of a given desaturase between the tumor area according to the Wilcoxon signed-rank test (p < 0.01).