Figure 8.

Representative immuno-Western blot of testicular StAR protein in control and hookah smoke (HS)-exposed group, with or without gum acacia (GA) treatment. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as internal control for the antibodies. The histograms represent the average means ± SEM of relative density of protein bands quantified from three independent experiments using different set of testes in each experiment. There was a significant decrease in the StAR protein in the tissue protein homogenate isolated from testes of mice exposed to HS, an action that was significantly reversed by GA concomitant treatment.