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. 2020 Apr 28;10(5):684. doi: 10.3390/biom10050684

Figure 5.

Figure 5

Alkaline phosphatase staining in 2D and 3D cultures. HFDPC were negatively stained for ALP activity in 2D cultures. The expression of ALP was recovered once encapsulated in RAD16-I 3D scaffolds. Human Foreskin Fibroblasts (HFF) were used as a negative control and HeLa cells as a positive control for ALP staining.