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Characterization of adipogenic phenotype of HFDPC and ADSC cultured in RAD16-I scaffold for 21 days. (a) FABP4 immunofluorescence of 3D constructs under adipogenic induction; (b) Nile red staining of 3D constructs under adipogenic induction; (c) FABP4 western blot of HFDPC and ADSC cultured in 2D cultures and in 3D RAD16-I scaffold under control and adipogenic medium. Vinculin was used as an internal control. The image is representative of three replicates.
