Figure 2.

Twenty-four hour B. burgdorferi co-culturing with HEK293 cells. Co-cultured HEK293 and B. burgdorferi were imaged using confocal microscopy. B. burgdorferi are observed to adhere to the outside of HEK293 cells at 24 h (A), while 3D imaging revealed that B. burgdorferi were internalized by HEK293 cells at 24 h (B). Scale bar is 15 µm. Depth coding showed the relative position of HEK293 cells to B. burgdorferi (C,D). All images taken at 1000× magnification. Scale bar is 5 µm.