Table 1.
Antireplicative actions of brassicasterol and brassicasterol in combination with acyclovir against herpes simplex virus type 1 compared to standard acyclovir along with cytotoxicity properties.
| Molecules | CC50 (μM) | IC50 (μM) | SI (CC50/IC50) |
|---|---|---|---|
| Brassicasterol | >50 | 1.2 ± 0.12 | >41.7 |
| Brassicasterol combined with ACV | >50 | 0.7 ± 0.24 | >71.4 |
| ACV (standard) | >50 | 2.1 ± 0.13 | >23.8 |
The acquired values are means ± standard deviation (SD) of three independent measurements assayed in duplicate. CC50: 50% cytotoxic concentration, IC50: 50% inhibitory concentration. CC50 and IC50 values were determined by nonlinear regressions of concentration-response curves. The differences between treatments with test molecules and the controls were analyzed using a one-way ANOVA test tracked by post-hoc comparison tests (Dunnett and Student–Newman–Kuels), where statistical significance was p < 0.05, SI: Selectivity index defined as the ratio CC50/IC50, ACV: Acyclovir. For performing the statistical analyses, PRISM software version 8.0 (GraphPad Software, Inc., La Jolla, CA, USA) was employed.