Table 2.
Nucleolytic stability of oligonucleotides tested in these studies. The values of the half-lives (T1/2) are given for the hydrolysis reactions carried out in the presence of svPDE.
| ASO | Sequence 5’ → 3’ | T1/2 (min) |
|---|---|---|
| 1 | 5’-d(TTT CTT TTC CTC C)-3’ | 14.5 |
| 2 | 5’-d(CTC CAG AGC CCG A)-3’ | 25.0 |
| 1a | 5’-d(UB TT CTT TTC CTC C)-3’ | 3.5 |
| 1b | 5’-d(TTT CTT TTC C UBC C)-3’ | 4.0 |
| 1c | 5’-d(UBTT CTT TTC CTCUB)-3’ | 20.0 |
| 2a | 5’-d(CUBC CAG AGC CCGA)-3’ | 3.5 |
| 2b | 5’-d(CTC CAG AGC CUBGA)-3’ | 4.0 |
| 1 | 5’-d(TTT CTT TTC CTC C)-3’ + FESAN (1 equiv.) | 4.8 |
| 1 | 5’-d(TTT CTT TTC CTC C)-3’ + FESAN (2 equiv.) | 3.8 |
| 1 | 5’-d(TTT CTT TTC CTC C)-3’ + FESAN (3 equiv.) | 3.5 |