Figure 3.

The effect of collagenase inhibition on osteoclastogenesis in calvarial bones. (A) Collagen sponges containing PBS, lipopolysaccharide (LPS), or Ro 32-3555 were surgically inserted onto mouse calvariae. At 5 days after stimulation, mice were sacrificed, and the calvarial bone sections were prepared and stained for TRAP-activities. Bone area on which collagen sponges were inserted was photographed. Scale bars indicate 200 mm and 50 mm in upper and lower panels, respectively. (B) Histomorphometry analyses were performed to examine osteoclast number (N. OC/B. Pm) and osteoclast size (OC Pm/N. OC). (C) Both osteoclast-positive and -negative resorption lacunae were measured to calculate eroded surface per bone surface (ES/BS). Data are mean ± SD obtained from 5 samples. **, p < 0.01 against vehicle-treated control.