Figure 1.

Inhibitory effect of pregnadiene-11-hydroxy-16α,17α-epoxy-3,20-dione-1 (PYED-1) on S. aureus biofilm formation. (A) Biofilms were quantified after crystal-violet staining. Values are presented as means ±SDs. Asterisks indicate statistically significant differences between the treated and untreated biofilms (*p < 0.05 and **p < 0.01, respectively). (B) Confocal laser scanning microscopy (CLSM) analysis of the biofilm formed by S. aureus ATCC 29213 in the absence (left upper panel) or presence of PYED-1, at the concentrations of 2 µg/mL (right upper panel), 4 µg/mL (left inferior panel), and 8 µg/mL (right inferior panel).