Figure 2.

Eradicating effect of PYED-1 on S. aureus preformed biofilm. Biofilm formed after 48 h in 96-well microplates was treated with different PYED-1 concentrations for 24 h at 37 °C under static conditions. (A) Biofilm biomass were measured by crystal violet (CV) staining. (B) Biofilm viability was measured by tetrazolium salt reduction (XTT) assay. Error bars represent the standard deviation (SD) of three independent experiments. Asterisks indicate statistically significant differences between the treated and untreated biofilms (**p < 0.01). (C) CLSM analysis of preformed S. aureus ATCC 29213 biofilm without treatment (left upper panel) or treated with PYED-1 at 16 µg/mL (right upper panel), 32 µg/mL (left inferior panel), and 64 µg/mL (right inferior panel) for 24 h.