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. 2020 May 8;8(5):113. doi: 10.3390/biomedicines8050113

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Examples of immunoreactivity for M2 receptors and Kir3.1 channel within the heart tissue (atria). Immunoreactivity was significantly increased for both ACh-associated centers and Kir3.1 channel, as demonstrated in the represented images. Results for female rats are demonstrated in section A, whereas for male rats they are demonstrated in section B. Pictures were taken at a magnification of ×200. Scale bar: 50 μm. Fold change in immunoreactivity for M2 receptors and Kir3.1 channel within the heart tissue (C). For female PTZ-kindled rats, there was a 2.1-fold change in M2 and a 6.1-fold change in the Kir3.1 channel when compared to the controls. On the other hand, for male PTZ-kindled rats, there was a 3.8-fold change in M2 receptors and a 3.7-fold change in the Kir3.1 channel when compared to the controls, * p < 0.05.