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. 2020 May 9;10(5):737. doi: 10.3390/biom10050737

Figure 5.

Figure 5

Flow cytometric analysis of Annexin V-FITC/PI double-labeled differentiated SH-SY5Y cells: (a) (−) control, (b) Aβ1-42 (20 µM), (c) memantine hydrochloride (MEM) (50 µM) + Aβ1-42, and (d) cHP4 (50 µM) + Aβ1-42. Quadrants were shown as Q1: necrosis, Q2: late apoptosis, Q3: living cells, and Q4: early apoptosis. The symbol (*) represents statistically significant difference (p < 0.01) of viable cell ratios compared to Aβ1-42 application. GraphPad Prism 7, Anova: Dunnett’s multiple comparison test was used to calculate the values: Aβ1-42 vs. (−) control: p < 0.0001, Aβ1-42 vs. MEM: p = 0.0002, and Aβ1-42 vs. GPE3: p < 0.0001.