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Effect of ipratropium, tiotropium, and glycopyrrolate on MPP+ uptake in EpiAirway™ and Calu-3 cells. Monolayers of EpiAirway™ and Calu-3 cells were washed with EBSS and incubated for 5 min, at the basolateral side, in EBSS containing [3H]MPP+ (50 µM; 2 µCi/mL) in the absence (none) or in the presence of 500 µM ipratropium, tiotropium, or glycopirrolate, as indicated. Bars represent the mean ± SEM of three independent determinations. * p < 0.05, ** p < 0.01 versus none.
