Figure 5.

Active compounds from anthelmintic screens using the MMV Pathogen Box. NTS: newly transformed schistosomula, xL3: exsheathed L3. Assay outline and citations for data sources: C. elegans growth/motility screen – automated quantification using the INVAPP system [135]. C. elegans motility screen – automated quantification using the WMicrotracker ONE system [163]. C. elegans viability screen – automated quantification of viability by differential absorption of the dyes DB-1 and propidium iodide [164]. E. multilocularis metacestode screen – damage was assessed by quantifying the activity of phosphoglucose isomerase released into the culture media [162]. F. hepatica screens – viability of metacercariae was assessed using a scoring system taking into account membrane damage and fluke translucency, and viability of adults was assessed using a scoring system taking into account worm motility, colour and rigidity [165]. H. contortus screen – automated quantification of motility of exsheathed L3 worms [129], S. haematobium and S. mansoni screens – activity was assessed using a scoring system taking into account motility and morphological/tegumental changes [161].