Table 3.

Open tools for high-throughput phenotypic screening of motility and viability and their use for anthelmintic discovery.

tool	validated with	source code/description (license)
WormScan [117] automated WormScan [118]	C. elegans high-throughput screen [119]	paper supporting information [117–118]
Lifespan Machine [120]	C. elegans lifespan analysis	Github [121] (GPLv3)
WormAssay [122] Worminator [123]	Brugia malayi (adults and microfilariae), Cooperia spp. L3, Dirofilaria immitis microfilariae, Schistosoma mansoni [122–123]	Github [124] (GPLv2 or later)
Cestode motility ImageJ macro [125]	Echinococcus multilocularis protoscoleces	paper supporting information [125]
Wiggle index ImageJ macro [126–128]	multiple high-throughput library screens with H. contortus [129–134]	paper supporting information [126]
INVAPP paragon [135]	library screens with T. muris and C. elegans [39,135–137]	Github [138] (MIT license)
CellProfiler [139] CellProfiler schistosome pipeline [140] CellProfiler WormToolbox [141]	S. mansoni thioredoxin glutathione reductase inhibitor screening [140], C. elegans live/dead high-throughput screening [141]	Github [142] (BSD license) CellProfiler published pipelines website [143]