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. 2020 May 16;10(5):770. doi: 10.3390/biom10050770

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Percentage of adherence to endothelial cells (ECs) of HNE-stimulated RBCs. (B) ICAM-1 expression in ECs co-incubated with HNE-stimulated RBCs: Incubation conditions and measurement of cell adherence or ICAM-1 expression were as reported in the Section 2.8. Controls were HNE-untreated RBCs. Values are means ± SD of six independent experiments carried out in triplicate. Bars with special characters are significantly different with p < 0.05 (ANOVA associated with Fisher’s correction).